Imaging

 
 Bio-Imaging Core Facility
 Outline of the facility
Polarization-rotating Total Internal Reflection Fluorescence Microscopy (pr-TIRF)
 pr-TRIF provides 4-type of microscopy
  – TIRF mode: This mode provides fast and high contrast imaging; however, TIRF can observe a thin region of
a specimen, usually less than 200 nm
– Epi mode: Basic fluorescence microscopy mode (resolution: ~250nm)
– Polarization microscope: This mode allows measuring anisotropy of fluorophores and combines with TIRF mode or Epi mode.
– Polarization-rotationg TIRF: This mode simultaneously provides super-resolution (~15nm) and quantitative orientation
information of the fluorophores which are two-same fluorophores.
 Confocal Microscope (LSM 7 DUO, Zeiss)
 LSM 7 DUO is an advanced confocal microscope and this is its first introduction in Korea. This microscope combines the point scanning mode (LSM 780) and the line-scanning mode (LSM 7 Live) providing high-contrast or high speed imaging capacity for live cell imaging.
  – LSM 780
The sensitivity of LSM 780 is quite outstanding. This equipment has the GaAsP detectors which achieve 45 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. Therefore LSM 780 mode provides accurate details and contrast-rich images of the specimens. In addition, LSM 780 allows multicolor imaging up to ten dyes and photon counting.

– LSM 7 Live
The time resolution of LSM 7 Live is quite outstanding (up to 120 frames per second). The scanning unit uses a laser beam with a rectangular cross-section to illuminate a whole line in the sample, instead of a single point and imaged by CCD detectors which is faster than PMT.

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