List of Instruments

  • Next Generation Sequencing System



Hiseq 2500 [Illumina / USA]

일루니마_하이식



Sequencing technology of the system offers a powerful new approach to next-generation sequencing applications such as whole genome sequencing (de novo & researching), whole exome sequencing, highly multiplexed PCR amplicon sequencing, targeted resequencing, transcriptome, small RNA, methylation profiling, ChIP-Seq and more. The Hiseq 2500 System is a powerful and efficient ultla-high-throughput sequencing system that supports the broadest range of applications and study sizes.


 

  • High Performance Benchtop Quadrupole FT/Orbitrap System



Q Exactive [Thermo Scientific / USA]
qe

This instrument is a high performance benchtop quadrupole FT/Orbitrap system based on the combination of the most advanced quadrupole technology combined with the Orbitrap analyzer technique. This benchtop LC-MS/MS system combines quadrupole precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver exceptional performance and versatility. The Q Exactive Mass Spectromater is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in proteomics.


– Mass Range: 50 to 6,000 m/z for MS scan and ~2,500 m/z for precursor selection

– Mass Resolution (FWHM): R= 140,000 for MS and MS/MS scans.

– Mass Accuracy with external calibration: < 3 ppm RMS for MS and MS/MS scans.

– Mass Accuracy with internal calibration: < 1 ppm RMS for MS and MS/MS scans.

– Useful Scan Rate: Up to 12 Hz at high resolution

– Multiplex-MS: up to 10 precursors/scan

– MS Sensitivity: 50 fg Buspirone on column S/N 100:1 using SIM

– MS/MS Sensitivity: sub-femtomol for peptides

– Dynamic Range in a single spectrum (S/N): > 5000:1

– Polarity Switching: One full cycle in < 0.4 sec (one scan in positive ion mode and one scan in negative ion mode)

– Electrospray ion source voltage: up to 8 KV


 

  • High Resolution Microarray Scanner



SureScan Microarray Scanner [Agilent Technologies / USA]
microarray


The SureScan Microarray Scanner is a laser induced fluorescence scanner designed to read microarrays printed on a standard 1 inch x 3 inch glass slides. It measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. This system produces accurate absolute and relative quantitation of signal levels over a range of more than six orders of magnitude, far exceeding the capabilities of other systems. This enhances data quality across a broad range of applications such as gene expression, miRNA profiling, array CGH, ChIP, and methylation assays.


array

 

  • Selective Plant Illumination Microscopy



Lightsheet Z.1 [Carl Zeiss / Germany]
microscopy

Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate optical resolution, but good optical sectioning capabilities and high speed. In contrast to epifluorescence microscopy only a thin slice (usually a few hundred nanometers to a few micrometers) of the sample is illuminated perpendicularly to the direction of observation. A second method uses a circular beam scanned in one direction to create the lightsheet. As only the actually observed section is illuminated, this method reduces the photodamage and stress induced on a living sample. Also the good optical sectioning capability reduces the background signal and thus creates images with higher contrast, comparable to confocal microscopy. Because LSFM scans samples by using a plane of light instead of a point (as in confocal microscopy), it can acquire images at speeds 100 to 1000 times faster than those offered by point-scanning methods.

1. Main system Module Lightsheet single side (Microscope)

1) Transmission contrast for overview

– IR LED illumination

– No Kohler illumination

– Zoom : 0.36x – 0.7x

2) Spectral range of detection

– 400 – 740nm

3) Field of view

– 60um to 2.8mm

4) Detection Zoom

– 0.36x – 2.5x (continuous)

5) Embedded specimen size

– From < 1um to 5mm

6) Light sheet thickness

– 2um – approx. 14um (488mm standard)

 

2. Detection module

1) sCMOS sensor, requires liquid cooling, up to two detection module

2) Pixel size : 6.5um

3) Max. pixel format : 1920 x 1920 (3.7 Mpixel)

4) Bit depth : 15bit

5) Max. frame rate : 30 frame per sec at 1000×1000 pixel, in continuous z-drive mode

6) Data acquisition rate : up to 150 Mbyte/sec

 

3.Incubation system

1) Heaing block

– Heating of sample chamber medium

– Room temperature to 42℃, 0.5°/min

2) Peltier block

– Heating and coolin gof sample chamber

– 10℃ to 42℃, up to 1.5°/min heating, up to 1.0°/min cooling

3) Temperature stability

– ±0.1℃

4) CO2-Module

– Requires CO2 supply, adjustable concentration

– 0% to 10%


 

  • Real-Time PCR Detection System



CFX384 Touch [Bio-Rad / USA]
rtpcr

A real-time PCR is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR). The CFX384 Touch Real-Time PCR Detection System combines the C1000 Touch™ Thermal Cycler with the CFX384™ Optical Reaction Module to bring ease of use and rapid data acquisition to researchers performing high-throughput real-time PCR in a 384-well format. The CFX384 Touch System builds on the precise thermal control of the C1000 Touch Thermal Cycler to deliver the most sensitive, reliable detection for real-time PCR applications. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX384 Touch System was designed to advance your qPCR experiment


rtpcr

 

  • Biomolecular Interaction Analysis System



Monolith NT [Nanotemper Technologies / Germany]
interaction_analysis

MicroScale Thermophoresis (MST) is a powerful technique for quantifying biomolecular interactions. By combining the precision of fluorescence detection with the flexibility and sensitivity of thermophoresis, MST provides a flexible, robust and fast way to measure molecular interactions. When performing a MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, fluorescent fusion proteins, or intrinsic fluorescence.

– Affinity range: 1nM to mM

– Volume per measurement: <4ul

– Concentration of fluorescent molecule: 10-9~10-3M

– Sample per run: 16 samples

– Molecular weight range (Da): 101-107

– Time for experiment & analysis: minutes

– Immobilization free in any buffer (including serum and cell lysate)

– Temperature control: 22 – 45 °C

– Fluorescence channels per instrument: 2 (Blue, Red or Green)


 

  • Luminescence in vivo Imaging System



Nightshade LB 985 [Berthold Technologies / Germany]
imaging_system

NightSHADE LB 985 is a modular plant imaging system for monitoring luminescence and fluorescence in tissues, seedlings and whole plants. Housed in an absolutely light-tight darkroom its heart is a cooled CCD camera which can be mounted on top or at the side. As the first imaging system fully dedicated to plant research it offers unique features.

imaging_sys


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